Neuroendocrine disruption in the shore crab Carcinus maenas: Effects of serotonin and fluoxetine on chh- and mih-gene expression, glycaemia and ecdysteroid levels.
Identifieur interne : 000048 ( Main/Exploration ); précédent : 000047; suivant : 000049Neuroendocrine disruption in the shore crab Carcinus maenas: Effects of serotonin and fluoxetine on chh- and mih-gene expression, glycaemia and ecdysteroid levels.
Auteurs : Alexandrine Robert [France] ; Tiphaine Monsinjon [France] ; Jean-Paul Delbecque [France] ; Stéphanie Olivier [France] ; Agnès Poret [France] ; Frank Le Foll [France] ; Fabrice Durand [France] ; Thomas Knigge [France]Source :
- Aquatic toxicology (Amsterdam, Netherlands) [ 1879-1514 ] ; 2016.
Abstract
Serotonin, a highly conserved neurotransmitter, controls many biological functions in vertebrates, but also in invertebrates. Selective serotonin reuptake inhibitors (SSRIs), such as fluoxetine, are commonly used in human medication to ease depression by affecting serotonin levels. Their residues and metabolites can be detected in the aquatic environment and its biota. They may also alter serotonin levels in aquatic invertebrates, thereby perturbing physiological functions. To investigate whether such perturbations can indeed be expected, shore crabs (Carcinus maenas) were injected either with serotonin, fluoxetine or a combination of both. Dose-dependent effects of fluoxetine ranging from 250 to 750nM were investigated. Gene expression of crustacean hyperglycemic hormone (chh) as well as moult inhibiting hormone (mih) was assessed by RT-qPCR at 2h and 12h after injection. Glucose and ecdysteroid levels in the haemolymph were monitored in regular intervals until 12h. Serotonin led to a rapid increase of chh and mih expression. On the contrary, fluoxetine only affected chh and mih expression after several hours, but kept expression levels significantly elevated. Correspondingly, serotonin rapidly increased glycaemia, which returned to normal or below normal levels after 12h. Fluoxetine, however, resulted in a persistent low-level increase of glycaemia, notably during the period when negative feedback regulation reduced glycaemia in the serotonin treated animals. Ecdysteroid levels were significantly decreased by serotonin and fluoxetine, with the latter showing less pronounced and less rapid, but longer lasting effects. Impacts of fluoxetine on glycaemia and ecdysteroids were mostly observed at higher doses (500 and 750nM) and affected principally the response dynamics, but not the amplitude of glycaemia and ecdysteroid-levels. These results suggest that psychoactive drugs are able to disrupt neuroendocrine control in decapod crustaceans, as they interfere with the normal regulation of the serotonergic system.
DOI: 10.1016/j.aquatox.2016.03.025
PubMed: 27060239
Affiliations:
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Havre</settlement></placeName></affiliation></author><author><name sortKey="Delbecque, Jean Paul" sort="Delbecque, Jean Paul" uniqKey="Delbecque J" first="Jean-Paul" last="Delbecque">Jean-Paul Delbecque</name><affiliation wicri:level="3"><nlm:affiliation>University of Bordeaux, CNRS UMR 5287, Institut de Neurosciences Cognitives et Intégratives d'Aquitaine (INCIA), Avenue des Facultés, F-33405 Talence Cedex, France.</nlm:affiliation><country xml:lang="fr">France</country><wicri:regionArea>University of Bordeaux, CNRS UMR 5287, Institut de Neurosciences Cognitives et Intégratives d'Aquitaine (INCIA), Avenue des Facultés, F-33405 Talence Cedex</wicri:regionArea><placeName><region type="region" nuts="2">Nouvelle-Aquitaine</region><region type="old region" nuts="2">Aquitaine</region><settlement type="city">Talence</settlement></placeName></affiliation></author><author><name sortKey="Olivier, Stephanie" sort="Olivier, Stephanie" uniqKey="Olivier S" first="Stéphanie" last="Olivier">Stéphanie Olivier</name><affiliation wicri:level="3"><nlm:affiliation>Normandy University, UNIHAVRE, UMR SEBIO, Environmental Stress and Aquatic Biomonitoring, 25 rue Philippe Lebon, F-76063 Le Havre, France.</nlm:affiliation><country xml:lang="fr">France</country><wicri:regionArea>Normandy University, UNIHAVRE, UMR SEBIO, Environmental Stress and Aquatic Biomonitoring, 25 rue Philippe Lebon, F-76063 Le Havre</wicri:regionArea><placeName><region type="region" nuts="2">Région Normandie</region><region type="old region" nuts="2">Haute-Normandie</region><settlement type="city">Le Havre</settlement></placeName></affiliation></author><author><name sortKey="Poret, Agnes" sort="Poret, Agnes" uniqKey="Poret A" first="Agnès" last="Poret">Agnès Poret</name><affiliation wicri:level="3"><nlm:affiliation>Normandy University, UNIHAVRE, UMR SEBIO, Environmental Stress and Aquatic Biomonitoring, 25 rue Philippe Lebon, F-76063 Le Havre, France.</nlm:affiliation><country xml:lang="fr">France</country><wicri:regionArea>Normandy University, UNIHAVRE, UMR SEBIO, Environmental Stress and Aquatic Biomonitoring, 25 rue Philippe Lebon, F-76063 Le Havre</wicri:regionArea><placeName><region type="region" nuts="2">Région Normandie</region><region type="old region" nuts="2">Haute-Normandie</region><settlement type="city">Le Havre</settlement></placeName></affiliation></author><author><name sortKey="Foll, Frank Le" sort="Foll, Frank Le" uniqKey="Foll F" first="Frank Le" last="Foll">Frank Le Foll</name><affiliation wicri:level="3"><nlm:affiliation>Normandy University, UNIHAVRE, UMR SEBIO, Environmental Stress and Aquatic Biomonitoring, 25 rue Philippe Lebon, F-76063 Le Havre, France.</nlm:affiliation><country xml:lang="fr">France</country><wicri:regionArea>Normandy University, UNIHAVRE, UMR SEBIO, Environmental Stress and Aquatic Biomonitoring, 25 rue Philippe Lebon, F-76063 Le Havre</wicri:regionArea><placeName><region type="region" nuts="2">Région Normandie</region><region type="old region" nuts="2">Haute-Normandie</region><settlement type="city">Le Havre</settlement></placeName></affiliation></author><author><name sortKey="Durand, Fabrice" sort="Durand, Fabrice" uniqKey="Durand F" first="Fabrice" last="Durand">Fabrice Durand</name><affiliation wicri:level="3"><nlm:affiliation>Normandy University, UNIHAVRE, Faculty of Science and Technics, 25 rue Philippe Lebon, F-76063 Le Havre, France.</nlm:affiliation><country xml:lang="fr">France</country><wicri:regionArea>Normandy University, UNIHAVRE, Faculty of Science and Technics, 25 rue Philippe Lebon, F-76063 Le Havre</wicri:regionArea><placeName><region type="region" nuts="2">Région Normandie</region><region type="old region" nuts="2">Haute-Normandie</region><settlement type="city">Le Havre</settlement></placeName></affiliation></author><author><name sortKey="Knigge, Thomas" sort="Knigge, Thomas" uniqKey="Knigge T" first="Thomas" last="Knigge">Thomas Knigge</name><affiliation wicri:level="3"><nlm:affiliation>Normandy University, UNIHAVRE, UMR SEBIO, Environmental Stress and Aquatic Biomonitoring, 25 rue Philippe Lebon, F-76063 Le Havre, France. Electronic address: thomas.knigge@univ-lehavre.fr.</nlm:affiliation><country xml:lang="fr">France</country><wicri:regionArea>Normandy University, UNIHAVRE, UMR SEBIO, Environmental Stress and Aquatic Biomonitoring, 25 rue Philippe Lebon, F-76063 Le Havre</wicri:regionArea><placeName><region type="region" nuts="2">Région Normandie</region><region type="old region" nuts="2">Haute-Normandie</region><settlement type="city">Le Havre</settlement></placeName></affiliation></author></analytic><series><title level="j">Aquatic toxicology (Amsterdam, Netherlands)</title><idno type="eISSN">1879-1514</idno><imprint><date when="2016" type="published">2016</date></imprint></series></biblStruct></sourceDesc></fileDesc><profileDesc><textClass></textClass></profileDesc></teiHeader><front><div type="abstract" xml:lang="en">Serotonin, a highly conserved neurotransmitter, controls many biological functions in vertebrates, but also in invertebrates. Selective serotonin reuptake inhibitors (SSRIs), such as fluoxetine, are commonly used in human medication to ease depression by affecting serotonin levels. Their residues and metabolites can be detected in the aquatic environment and its biota. They may also alter serotonin levels in aquatic invertebrates, thereby perturbing physiological functions. To investigate whether such perturbations can indeed be expected, shore crabs (Carcinus maenas) were injected either with serotonin, fluoxetine or a combination of both. Dose-dependent effects of fluoxetine ranging from 250 to 750nM were investigated. Gene expression of crustacean hyperglycemic hormone (chh) as well as moult inhibiting hormone (mih) was assessed by RT-qPCR at 2h and 12h after injection. Glucose and ecdysteroid levels in the haemolymph were monitored in regular intervals until 12h. Serotonin led to a rapid increase of chh and mih expression. On the contrary, fluoxetine only affected chh and mih expression after several hours, but kept expression levels significantly elevated. Correspondingly, serotonin rapidly increased glycaemia, which returned to normal or below normal levels after 12h. Fluoxetine, however, resulted in a persistent low-level increase of glycaemia, notably during the period when negative feedback regulation reduced glycaemia in the serotonin treated animals. Ecdysteroid levels were significantly decreased by serotonin and fluoxetine, with the latter showing less pronounced and less rapid, but longer lasting effects. Impacts of fluoxetine on glycaemia and ecdysteroids were mostly observed at higher doses (500 and 750nM) and affected principally the response dynamics, but not the amplitude of glycaemia and ecdysteroid-levels. These results suggest that psychoactive drugs are able to disrupt neuroendocrine control in decapod crustaceans, as they interfere with the normal regulation of the serotonergic system.</div></front></TEI><affiliations><list><country><li>France</li></country><region><li>Aquitaine</li><li>Haute-Normandie</li><li>Nouvelle-Aquitaine</li><li>Région Normandie</li></region><settlement><li>Le Havre</li><li>Talence</li></settlement></list><tree><country name="France"><region name="Région Normandie"><name sortKey="Robert, Alexandrine" sort="Robert, Alexandrine" uniqKey="Robert A" first="Alexandrine" last="Robert">Alexandrine Robert</name></region><name sortKey="Delbecque, Jean Paul" sort="Delbecque, Jean Paul" uniqKey="Delbecque J" first="Jean-Paul" last="Delbecque">Jean-Paul Delbecque</name><name sortKey="Durand, Fabrice" sort="Durand, Fabrice" uniqKey="Durand F" first="Fabrice" last="Durand">Fabrice Durand</name><name sortKey="Foll, Frank Le" sort="Foll, Frank Le" uniqKey="Foll F" first="Frank Le" last="Foll">Frank Le Foll</name><name sortKey="Knigge, Thomas" sort="Knigge, Thomas" uniqKey="Knigge T" first="Thomas" last="Knigge">Thomas Knigge</name><name sortKey="Monsinjon, Tiphaine" sort="Monsinjon, Tiphaine" uniqKey="Monsinjon T" first="Tiphaine" last="Monsinjon">Tiphaine Monsinjon</name><name sortKey="Olivier, Stephanie" sort="Olivier, Stephanie" uniqKey="Olivier S" first="Stéphanie" last="Olivier">Stéphanie Olivier</name><name sortKey="Poret, Agnes" sort="Poret, Agnes" uniqKey="Poret A" first="Agnès" last="Poret">Agnès Poret</name></country></tree></affiliations></record>Pour manipuler ce document sous Unix (Dilib)
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